Thi Van Nguyen 1, Yuna Jiang 1, Zhen Nan Yu 1, Juan Jin 1, Eui Hyeog Han 1, Hyoung Tae Kim 1, Chang Ho Song 1,2, Yong Kap Hur 3, Ok Hee Chai 1,2
1Department of Anatomy, Jeonbuk National University Medical School, Jeonju, Republic of Korea
2 Institute of Medical Science, Jeonbuk National University, Jeonju, Republic of Korea
3Uniquebiotech, Co., Ltd., Iksan, 54576, Republic of Korea
INTRODUCTION
Mast cells play a critical role in the effector phase of immediate hypersensitivity and allergic reactions. Propolis exerts multiple biological effects and exhibits immunomodulatory and antioxidant properties. However, its role in dinitrophenyl (DNP) immunoglobulin E (IgE)-activated mast cells has not been investigated. In this study, we examined the mechanism underlying the inhibitory effect of propolis on anti-DNP IgE-mediated mast cell degranulation, with a specific focus on histamine and cytokines release using rat peritoneal mast cells (RPMC).
MATERIALS AND METHODS
1. Reagent: anti-DNP IgE, DNP-HSA (sigma), propolis (Unibee powder propolis, Uniquebiotech, Co., Ltd.,Iksan)
2. RPMC viability assay
3. ELISA assay: Measure the level of histamine, IL-6,TNF-𝛼, IL-1β, IL-4, IL-5, IL-13.
4. Western blot: Erk, JNK, p-38 and their phosphorylation form; LAT, PLCγ1 and their phosphorylation form.
CONCLUSION
Propolis protected mast cell degranulation and inhibited histamine release from RPMC triggered by anti-DNP IgE in vitro. Interestingly, propolis also decreased the levels of TNF-alpha, IL-4, IL-6, and IL-13 released from anti-DNP IgE-activated RPMC. Moreover, the MAPK/SYK signaling pathway also was suppressed in the anti-DNP IgE activated RPMC by propolis. Together, these results suggest that propolis effectively inhibits IgE-mediated mast cell anaphylaxis-like reactions.
Korean Association Anatomists. 2022 Annual meeting. 72
Thi Van Nguyen 1, Yuna Jiang 1, Zhen Nan Yu 1, Juan Jin 1, Eui Hyeog Han 1, Hyoung Tae Kim 1, Chang Ho Song 1,2, Yong Kap Hur 3, Ok Hee Chai 1,2
1Department of Anatomy, Jeonbuk National University Medical School, Jeonju, Republic of Korea
2 Institute of Medical Science, Jeonbuk National University, Jeonju, Republic of Korea
3Uniquebiotech, Co., Ltd., Iksan, 54576, Republic of Korea
INTRODUCTION
Mast cells play a critical role in the effector phase of immediate hypersensitivity and allergic reactions. Propolis exerts multiple biological effects and exhibits immunomodulatory and antioxidant properties. However, its role in dinitrophenyl (DNP) immunoglobulin E (IgE)-activated mast cells has not been investigated. In this study, we examined the mechanism underlying the inhibitory effect of propolis on anti-DNP IgE-mediated mast cell degranulation, with a specific focus on histamine and cytokines release using rat peritoneal mast cells (RPMC).
MATERIALS AND METHODS
1. Reagent: anti-DNP IgE, DNP-HSA (sigma), propolis (Unibee powder propolis, Uniquebiotech, Co., Ltd.,Iksan)
2. RPMC viability assay
3. ELISA assay: Measure the level of histamine, IL-6,TNF-𝛼, IL-1β, IL-4, IL-5, IL-13.
4. Western blot: Erk, JNK, p-38 and their phosphorylation form; LAT, PLCγ1 and their phosphorylation form.
CONCLUSION
Propolis protected mast cell degranulation and inhibited histamine release from RPMC triggered by anti-DNP IgE in vitro. Interestingly, propolis also decreased the levels of TNF-alpha, IL-4, IL-6, and IL-13 released from anti-DNP IgE-activated RPMC. Moreover, the MAPK/SYK signaling pathway also was suppressed in the anti-DNP IgE activated RPMC by propolis. Together, these results suggest that propolis effectively inhibits IgE-mediated mast cell anaphylaxis-like reactions.
Korean Association Anatomists. 2022 Annual meeting. 72